Intrathecal injection of ozone alleviates CCI‑induced neuropathic pain via the GluR6‑NF‑κB/p65 signalling pathway in rats

Ozone is widely used to relieve chronic pain clinically, but the precise mechanisms governing its action have yet be elucidated. The present study aimed investigate underlying pain‑alleviating effect of ozone in constriction injury (CCI) model sciatic nerve rats. Pain behaviours rats were assessed by mechanical allodynia and thermal hyperalgesia. expression spinal glutamate receptor 6 (GluR6) NF‑κB/p65 was detected western blotting reverse transcription‑quantitative PCR. Meanwhile, IL‑1β, IL‑6 TNF‑α ELISA. GluR6 short interfering (si)RNAs intrathecally immediately following CCI once per day. (10, 20 or 30 µg/ml) oxygen injected on day 7 after CCI. level increased 3 reached a peak IL‑6, also In addition, pre‑intrathecal injection siRNAs inhibited suppressed GluR6, 7. Intrathecal observed inhibit suppress suggested that served pivotal role neuropathic intrathecal may alleviate via GluR6‑NF‑κB/p65 signalling pathway.